A comprehensive approach to the in vitro and in vivo study of a preparation containing etidronate of lanthanide and calcium ions

Boichuk S.V., Zhitlova E.A., Shakirova F.V., Tsyplakov D.E., Akhtiamov I.F., Deviatov F.V., Ramazanov B.R., Zakirov R.K.


The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare.
To explore the efficacy of a preparation containing etidronates of lanthanide and calcium ions in regenerative repair of bone defects.
Material and methods
The osteoblastic MC3T3-E1 and the osteoclastic RAW 264.7 cell lines were used in in vitro experiments at the first stage of the research. The agent was postoperatively injected in a bone defect of 36 rabbits on days 3 and 5 to assess the preparation’s effect on regenerative repair of small defects with diameter of 2.5 mm. Radiometric and reactive morphological characteristics of bone tissue were evaluated at the fracture site at the beginning, middle and end of experiment.
The above preparation was found to enhance osteogenic differentiation and stimulate accumulation of intracellular calcium in MC3T3 E1 cells. However, the preparation was not shown to inhibit RANKL-induced osteoclast differentiation in vitro. Histological and computed tomography findings demonstrated statistically significant differences between control and experimental animals (p less 0.01) and indicated to the preparation’s effect of promoting regenerative repair of small bone defects.
The series was the first to show the effect of the preparation containing etidronates of lanthanide and calcium ions as stimulating osteoblast activity in vitro and promoting early regenerative repair of small bone defects.


lanthanide, etidronate, osteoblast, osteoclast, osteolysis, regeneration of bone defect

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DOI: http://dx.doi.org/10.18019/1028-4427-2019-25-4-561-568


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